Dear All,
Apart from ImageJ, which software do you use to analyse light microscopy images?
To do basic things like colocalization analysis, measure of fluorescence increase/decrease against time (Ca2+ recording for example), counting the number of fluorescent events against time etc?
Thank you!
For whom it may be of interest, here is a very helpful answer from Krunalkumar Shah:
There are several software options available for analyzing fluorescent imaging data apart from ImageJ. Here are a few popular ones: 1. **FIJI/ImageJ**: Although you mentioned excluding it, ImageJ's extended version FIJI is worth considering due to its wide user base and extensive plugin library specifically designed for image analysis. 2. **CellProfiler**: This is a free, open-source software designed for high-throughput image analysis. It's particularly useful for tasks like cell counting, object identification, and intensity measurements. 3. **Imaris**: Imaris is a powerful software suite for visualizing, analyzing, and interpreting 3D and 4D microscopy data. It's commonly used for tasks like colocalization analysis, tracking objects over time, and quantifying fluorescence intensity. 4. **Volocity**: Volocity is another software package designed for 3D and 4D image analysis. It offers features for colocalization analysis, object tracking, and measurement of cellular dynamics. 5. **MetaMorph**: MetaMorph is a versatile software platform that supports a wide range of microscopy applications, including fluorescence imaging. It provides tools for image analysis, object tracking, and time-lapse analysis. 6. **CellProfiler Analyst**: This is an extension of CellProfiler designed specifically for machine learning-based analysis of large image datasets. It's useful for tasks like classification, clustering, and data exploration. 7. **Huygens Software**: Huygens is known for its advanced deconvolution algorithms, making it suitable for improving image quality in fluorescence microscopy. It also offers tools for image analysis and visualization. Each software has its strengths and may be better suited to specific types of analysis or workflows. It's often helpful to try out a few options to see which one fits your needs best. Many of these software packages offer free trials or open-source versions, so you can explore them before making a decision.
Hey Lora,
using VAIDR can help.
You can train the AI on the platform to your needs.
Analyze images from any microsope with AI (youtube.com)
& read here: www.vaidr.de
You can get a test account - let me know! -> [email protected]
This is free of cost. Using VAIDR is intuitive. You do not need to have AI skills.
Best regards,
Thomas