15 September 2018 0 8K Report

En nuestra última sesión de seguimiento, se me señaló que era importante incluir a las autoras más recientes sobre la participación de la mujer en los medios de comunicación.

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Why is my protein assay standard curve bended?

Hello everyone, I wish to measure the total protein concentration of some samples but my standards are always bended rather than giving a nice straight line. I first tried to make the standards...

09 August 2023 3,305 7 View

Oxytocin plasmids for cell transfection?

Dear All, I am currently looking for a plasmid (or paper using a plasmid) of oxytocin to transfect it to cell lines. On Addgene, I only found viral plasmids and if I can avoid that, that would...

05 June 2023 8,658 0 View

The results of measuring of MMP with TMRM not reproduced?

Hi everybody! I've been analyzing mitochondrial membrane potential (ΔΨm) with TMRM in HEK293T cells at 20nM for 20 minutes in the HBSS buffer. However, from experiment to experiment, I get...

03 October 2021 4,130 2 View

How can I increase the signal of the 4 ng band in Non-reduced Licor -Odyssey western blot?

I load 4ng and then 200ng protein in two adjacent wells. 4ng protein (conc. 0.8mg/ml) is derived from a two step serial dilution of 2mg/ml protein. Proteins are transferred to Fluorescent...

12 August 2021 8,681 4 View

Does multiple stimulation with DHPG are effective ?

Hi everyone ! I am performing acute slice Ca-imaging and I would like to register the fluo rate increase on neurons in response to DHPG( group I mGlu receptor agonist). Does somenone know if I...

25 February 2019 7,932 1 View

Identify oxytocin neuron in living hypothlamaic slices ?

Hello, does anyone know a way to identify oxytocin neuron over the vasopressin neuron in acute slices ? I am performing calcium imaging in the PVN of mice with Calbryte dye and recording calcium...

19 November 2018 9,653 3 View

Hi, does dissolving polar compounds in water change its dielectric constants/polarity?

compounds like amino acids or nicotinamide. thank you

17 October 2018 5,102 4 View

How to measure the solubility of one compound in a mixture of organic compounds?

 (ethyl acetate)

18 August 2017 2,022 3 View

Which is better for cell lysis preparation for ELISA: Triton X100 or Tween 20? What is the percentage that needs to be used?

I am preparing cell samples for ELISA to measure intracellular (total) protein expression. I do not want to extract the proteins as this is not compatible with the kit we have available. I want to...

15 March 2017 1,855 3 View

How effective are global health interventions in developing countries?

I am researching for effective models and approaches to deliver effective and sustainable improvements. Consulting by experts to identify and solve problems, projects to deliver services to the...

23 June 2016 6,446 6 View

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