Identify oxytocin neuron in living hypothlamaic slices ?

Hello, does anyone know a way to identify oxytocin neuron over the vasopressin neuron in acute slices ? I am performing calcium imaging in the PVN of mice with Calbryte dye and recording calcium...

19 November 2018 9,528 3 View

How to measure the solubility of one compound in a mixture of organic compounds?

18 August 2017 1,862 3 View

Which is better for cell lysis preparation for ELISA: Triton X100 or Tween 20? What is the percentage that needs to be used?

I am preparing cell samples for ELISA to measure intracellular (total) protein expression. I do not want to extract the proteins as this is not compatible with the kit we have available. I want to...

15 March 2017 1,729 3 View

How effective are global health interventions in developing countries?

I am researching for effective models and approaches to deliver effective and sustainable improvements. Consulting by experts to identify and solve problems, projects to deliver services to the...

23 June 2016 6,312 6 View

Does anyone know how to set the Custom Field Functions in FLUENT?

Hello everyone, I have few questions : I'm newbie in ANSYS Fluent (CFD simulation).I want to make a simulation of Pool Boiling using PCB as a heater.Here is my geometry. I set up the heater's...

22 November 2015 3,947 3 View

What is the best vector for a TetON system with a bidirectional promoter?

I cloned 2 genes of my interest into the pBI-4 vector, so that I could activate the expression of both genes at the same time. However, the construct is quite leaky, so that I get expression of...

04 June 2014 2,144 2 View

What is the best way to transfect HUVECs?

I tried Metafectene, peqFECT and Fugene. Nothing worked so far.

02 October 2013 1,371 11 View

Markers to detect human or mouse myeloid suppressor cells (MSDC) by Immunohistology and flow cytometry

I need antibodies to detect MSDC by immunohistology and flow cytometry in cancer infiltrate.

23 January 2013 8,993 3 View

Markers to detect human or mouse myeloid suppressor cells (MSDC) by Immunohistology and flow cytometry

I need antibodies to detect MSDC by immunohistology and flow cytometry in cancer infiltrate.

31 December 2012 9,241 3 View

Vasopressin half-life

Hello everyone, By searching the half-life of vasopressin, it appears that there are huge fluctuations between studies... Some demonstrate that, in blood, the half-life is about 2-3 min whereas...

01 January 1970 4,318 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How to find natural frequency of a bridge from vehicle induced vibrations?

i have frequencies of bridge that are effected by mass of vehicle. the problem is when any vehicle pass the bridge it would add the its mass to the bridge mass ultimately mass of the bridge will...

22 February 2021 10,004 3 View