Hi, does anyone have an immunohistochemical protocol for detecting connexin 43 in zebrafish myocardium?

21 November 2017 0 398 Report

Hi, does anyone have an immunohistochemical protocol for detecting connexin 43 in zebrafish myocardium? I would like to know how the antigenic recovery was done and which buffer used for sample washing (paraffin embedded tIssue).

Badges
Science topic

More Marcela Aldrovani's questions See All

No title

It was published in the Journal of the American College of Toxicology in 1990, but the author's information does not appear in the journal

29 November 2020 2,554 3 View

Has anyone been successful in seeding microglia from adult mice?

Hello everybody I've been trying to seed microglia from adult mice and I haven't been successful. I isolated them using Miltenyi Beads and that worked perfectly, passed them through the...

14 October 2020 4,572 3 View

Can I use the cryostat to slice brains that were perfused with 4% PAF and Gadoteridol?

Hi everyone Im trying to slice my mice brains with the cryostat, however, I haven't been successful, the tissue seems to stretch and then it breaks. Let me tell you the story about these brains:...

25 August 2020 6,479 4 View

How to transfect Tcells with siRNA?

Hi everyone, I am trying to transfect murine CD4+ T cells in vitro, using Lipofectamine RNAi/MAX however, even when trying troubleshoot, the positive FITC labelled control shows just about 3%...

03 June 2020 2,618 1 View

Alternate morphology of eletrocardiographic signals after heart mechanical injury..... ???

Hello, I need your help. We are studying electrocardiographic parameters in an experimental model of heart injury, and we obtained unexpected results. The ECG intervals alternated during the...

28 September 2017 2,581 4 View

What can I do to avoid contamination of my nested pcr?

Good day, I try to make a pcr nested but I have problems of contamination, I use as a template the negative product of the pcr in the second pcr and gives me positive. I thought that the pollution...

24 January 2017 6,992 2 View

Is there any reliable antibody against corticotropin-releasing hormone (CRH) for Western blot?

Hi guys, does anybody know any CRH antibody which would work for western blots giving a nice and specific signal, especially in brain tissues (PVN, hippocampus)? Any advice is welcome. Thank you.

27 January 2016 320 3 View

Sophie's choice: Random mate-pairs or gel selection in Illumina Nextera mate-pair preparation?

Hi guys! I'm sequencing and assembling a de novo invertebrate genome of about 0,8Gb. I want to make a lane run of Illumina nextera mate-pairs, but I'm having some difficulty on selecting the size...

06 January 2015 6,134 9 View

Can anybody recommend a good and specific p-CREB and CREB antibody for westerns in rat brain tissue samples?

Thanks in advance.

22 February 2014 7,262 4 View

The most appropriate nuclear and cytosolic fraction housekeeping gene?

Could anyone recommend the most appropriate housekeeper for nuclear and cytosolic fraction? I want to isolate both fractions from frozen rat brain tissues and am wondering what is the best and...

05 August 2013 8,453 6 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View