Hi, does anyone have an immunohistochemical protocol for detecting connexin 43 in zebrafish myocardium? I would like to know how the antigenic recovery was done and which buffer used for sample washing (paraffin embedded tIssue).
So through social media, advertising, politics and life in general. I know the Stroop Effect is a psychological and biological effect but I am interest to search if, and if yes, it can be present...
13 May 2024 8,371 2 View
The one in Open Biosystems does not work anymore. Thank you!
02 July 2023 1,187 0 View
Hello! Im working with primary human MSC, I obtained them from adipose tissue, however, I've been trying to expand them unsuccessfully, I use normal T-75 tissue culture flask, DMEM with 15% SFB,...
22 June 2023 6,154 2 View
Hello everyone I'm working with ATCC-BYS0111 Human [Hispanic Male] Induced Pluripotent Stem (IPS) Cells ACS-1025™ and the thing is that their morphology is changing a lot, I've been following the...
07 November 2022 3,632 4 View
I'm trying to download the CPCe software (Coral Point Count with Excel extensions) to analyze some images for my thesis but without success. I am always redirected to the NSU Florida website...
04 November 2022 8,217 2 View
Hi, I want to esterify triglycine, the methodology I'm trying to follow indicates the use of thionyl chloride reagent. The thing is this is not sold in my country so I'm looking for a substitute....
24 October 2022 9,782 6 View
Hello everyone I've got an IBox machine that I'd like to use, however, I was wondering if any of you has used it ex vivo? I mean, I've got a breast cancer mouse model where I'm studying metastasis...
17 October 2022 4,348 0 View
Hello everyone, I am trying to stain macrophages in skin wound healing tissue, and it's been very tricky to establish a proper protocol. I tried markers F4/80 and MOMA-2, and Cy3 as fluorophore...
15 August 2022 7,587 0 View
It was published in the Journal of the American College of Toxicology in 1990, but the author's information does not appear in the journal
29 November 2020 2,851 3 View
Hello everybody I've been trying to seed microglia from adult mice and I haven't been successful. I isolated them using Miltenyi Beads and that worked perfectly, passed them through the...
14 October 2020 4,792 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...
05 August 2024 8,056 1 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View