Hello everybody
I've been trying to seed microglia from adult mice and I haven't been successful.
I isolated them using Miltenyi Beads and that worked perfectly, passed them through the cytometer and confirmed my isolation worked good and about 85% were viable. I seeded them in 24-well plates with Poly-L-Lysine but microglia didn't make it throughout the night.
Any suggestions?
Thank you
Hi Marcela,
we successfully isolated microglia from the brain and cultured them overnight for in vitro experiments. We also uses CD11b-Bead sorting from Milteny. We did not use however coated plates but untreated ones.
If you like have a look at our paper. In the Materials section under "Isolation, culture, and stimulation of primary microglia" you find the brief protocol.
Article The TSPO-NOX1 axis controls phagocyte-triggered pathological...
For any more detailed questions you can also as Anne Wolf here on ResearchGate. She is the specialist.
All the best and stay healthy,
Marc
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