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It was published in the Journal of the American College of Toxicology in 1990, but the author's information does not appear in the journal

29 November 2020 2,554 3 View

Has anyone been successful in seeding microglia from adult mice?

Hello everybody I've been trying to seed microglia from adult mice and I haven't been successful. I isolated them using Miltenyi Beads and that worked perfectly, passed them through the...

14 October 2020 4,572 3 View

Can I use the cryostat to slice brains that were perfused with 4% PAF and Gadoteridol?

Hi everyone Im trying to slice my mice brains with the cryostat, however, I haven't been successful, the tissue seems to stretch and then it breaks. Let me tell you the story about these brains:...

25 August 2020 6,479 4 View

How to transfect Tcells with siRNA?

Hi everyone, I am trying to transfect murine CD4+ T cells in vitro, using Lipofectamine RNAi/MAX however, even when trying troubleshoot, the positive FITC labelled control shows just about 3%...

03 June 2020 2,618 1 View

What is the difference between KPIs and Balanced Scorecard ?

Measuring business performance Organisations face the intense pressure of carrying out their processes efficiently, effectively and sustainably every day (Ghosh & Fedorowicz, 2008). Companies...

30 March 2020 1,553 4 View

Ghost signals in LC/MS scan mode. Contamination?

Dear experts across the world, today I am looking for advice on a new field my lab is starting to work on: LC/MS. We are using an Agilent 1260 Infinity system, with a 6120 Quadrupole LC/MS. We do...

17 December 2018 6,718 3 View

Is there anyone that has used Electron Microscopy Sciences grids for EV analysis?

Hi everybody. I wonder if there is someone here that has successfully worked with TEM grids sold by Electron Microscopy Sciences for the analysis of extracellular vesicles (microvesicles and...

13 November 2018 9,993 1 View

How can I avoid my mice being so aggressive among each other?

Hello everybody I'm currently trying to work with aging, and for that, I'm letting my mice become older (males, C57/BL6). The thing is, they literally are eating themselves off, I've tried...

02 March 2018 1,937 12 View

How to compare 2 variables where 1 has 2 possible values and the other one are experimental data?

I made 30 assays testing the capacity of 2 chemical products in the removal percentage of dirt. 15 assays were made with the chemical product `0` and 15 with the chemical product 1. I obtained a...

23 November 2017 8,831 1 View

How to compare a 3 sets of data in order to sort out how 2 of these data influence the third one?

Hi, I have a 3 set data like the image There is a tool to say what is the most important variable in the removal, pH or dosage? I was thinking in a PCA (principal component analysis) however I'm...

10 November 2017 6,864 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View