I borrowed a bottle of HEK293T cell culture from someone else, but after one passage, the cell morphology became very strange and not round at all. May I ask why? We used T25 culture flasks and digested them with 500 microliters of pancreatic enzyme at 37 degrees Celsius for 30 seconds.
Dear Siyu
What has been the FBS concentration? I recommend to elevate the FBS percentage up to 12%. I think that this morphology is not peculiar for HEK cells when the cell density is low.
Dear colleague, it is quite obvious from your micrograph that almost all the cells in your culture have obvious morphological signs of apoptosis. Consequently, the culture as a whole has experienced some lethal impact (infection, toxin, untimely passage, etc.).
Alexey Orlovsky added an answer
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