We are having a problem in detecting the basal eNOS levels in freshly isolated PBMCs by RT-PCR or western blotting. Does anyone have suggestions for the same and a maybe a protocol?
Well, I must say that you should check or you may be interested in checking the expression of iNOS but not eNOS. As eNOS is expressed in endothelial cells or blood capillary cells however, PBMCs may have iNOS expression if activated due to any external stimulus e.g. inflammation. So, please re-analyze the problem which you have chosen to work with. Good luck.