I need to immobilize several polysaccharides (mainly glucans) on microplate wells for further treatment and analysis by fluorescence. Any suggestions?
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...
02 March 2021 1,830 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...
01 March 2021 4,128 3 View
I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??
28 February 2021 2,153 1 View
Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.
28 February 2021 3,640 3 View
The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...
28 February 2021 6,560 14 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?
24 February 2021 8,219 2 View
what all standards are needed to quantify and analyze the composition with a proper method fie for the mobile phase to run the sample ?
31 January 2021 7,704 4 View
I 'm trying to extract polysacharides from Aloe leaf and tried ethanol percipitation, but after getting the pellet. I used the protocol from following reference following the steps, but I don't...
27 January 2021 7,049 5 View
I am working on heavy metal bioremediation using bacterial isolates. I have immobilized bacteria in 2% sodium alginate then cross-linked in 0.2M calcium chloride for 30 min. I have successfully...
04 January 2021 9,094 4 View
Hi! We are aiming to bind streptavidin to polyacrylamide surface. The EDC/NHS method bounced on me as a common method, utilizing the -NH2 of polyacrylamide and -COOH of streptavidin. Is there any...
17 December 2020 7,944 2 View
i am performing SPR experiment and i am referring some journal and websites they stated regarding coupling buffer and immobilization buffer so ihave confusion about these buffer.
06 December 2020 470 3 View
Dear research community. I want to study FTIR spectra of Immobilized enzyme on alginate beads before and after biodegradation, do i need to grind the Immobilized enzyme on alginate beads for the...
26 October 2020 8,606 1 View
Hi, I have this "bad" DNA extract of a Fucus spp. and I want to purifying this because the PCR analysis that I need don't show results. I alredy try to extract twice the DNA both with the Genomic...
06 October 2020 1,500 1 View
I immobilize fluorescent peptide on chitosan films and when I try to measure the thickness of the film with ellipsometry, the software says that I have a film with 2 nm or less. However, with...
01 October 2020 3,702 3 View
We are looking to immobilize some of our enzymes on Eupergit beads. I was wondering if you know any commercial supplier of Eupergit beads?
28 September 2020 5,664 3 View
Actually I have faced a significant quantity of nanocatalyst loss during immobilization on glass surface. Though I have used ethanol(volatile) to spread the catalyst effectively towards the...
22 September 2020 4,551 8 View