I am working on research that involves imaging of giant unilamellar vesicles (GUVs) using SRS microscopy. When I try to image GUVs using SRS, the GUVs would move around, which make it very difficult to image them and I am looking for a way to immobilize them on glass slides. I tried a method that utilizes biotin-avidin interaction to immobilize them but I was not able to immobilize them successfully. I even had trouble making GUVs using biotinylated lipids. Has anyone worked with GUVs or something similar and had similar issues? If so, what methods did you use to immobilize them? Thank you so much for your help.
If you are not doing membrane dynamics studies, then you can go for agarose immobilization. For other cases, you can also immobilise using BSA coating on cover slides.
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