Hello all,
I am culturing macrophages to differentiate and polarize them in vitro. I need to detach them from the plastic dishes for flow cytometry characterization, but I am having trouble with that part.
I started using trypsine, but I am afraid that it may damage the surface markers. I then tried the PBS/EDTA/Cold protocols suggested on the different discussions on this network, but it did not work for me. It takes forever, I loose many cells that still stay on the dish, and viability afterwards is very low.
My next option was to try TrypLE Select from Gibco, because we have it in the lab, but we have no experience with this in macrophages. Can someone give me an advice on this, please?
Thanks!
Hello Ines, after I washed Bone marrow macrophages BMM cultured plates with pre-warmed medium 3 times by pipetting, I added medium and used a cell scraper to detach them. I performed flow citometry and got well.
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