Ines Maldonado-Lasuncion

2 Questions 3 Answers 0 Followers

Questions related from Ines Maldonado-Lasuncion

My macrophages do not proliferate well after detaching and splitting, any advice?

Hello, I am having trouble obtaining large amounts of macrophages in culture. I obtain them from SD rats bone marrow and sort them before culture. I let the sorted monocytes sit for 7 days (adding...

21 September 2017 1,571 4 View

Has someone used TrypLE Select to detach macrophages?

Hello all, I am culturing macrophages to differentiate and polarize them in vitro. I need to detach them from the plastic dishes for flow cytometry characterization, but I am having trouble with...

22 May 2016 9,915 1 View