I have transfected L929 cells with polio virus in T-75 flask. I left it for 48 hours and now I collected the media which is 15ml DMEM. I want the best way to isolate the viral RNA from this media.
I was thinking, on such a large scale, no RNA extraction kit would help, so I thought of a sucrose cushion, or the centrifugal concentrator. The centrifugal concentrator is easier to use, but I am not sure how much I am going to lose of the virus in the media.