Pe28a+ -Kanamaycin resistant, no clones after transformation to E coli competent?

I am transforming 3 different vectors in E coli competent bacteria: cDNA3.1, Pe16b and Pe28a+. I used the same protocol (heat shock) for transformation and plating ( agr + appropriate...

11 December 2019 3,238 2 View

Is their any relation between modic changes of vertebra and diabetes?

I need to know if their is any relation between modic changes in the vertebra and diabetes. And if any one aware of any paper in literature regarding that

23 March 2019 8,331 4 View

What are the real indication of OLIF a Nd DLIF in spine surgery?

08 June 2016 9,438 3 View

How can i plot Cp or Nu around a circle in CFD-Post or fluent?

Hello guys, i have been working on this problem for quite some time now, i was able to plot Nu vs theta using poly lines,expressions and variables however the plot does not start from 0 degrees...

11 February 2016 195 6 View

How can I recover protein sample from RIPA buffer?

16 October 2015 798 13 View

Has anyone used centrifugal concentrator tubes?

I have transfected L929 cells with polio virus in T-75 flask. I left it for 48 hours and now I collected the media which is 15ml DMEM. I want the best way to isolate the viral RNA from this media....

06 October 2013 6,929 4 View

Quality of the training environment

Quality of the training environment is new term, Includes all appliances, tools and sports fields, and the impact on the psychological athlete. who can give helpful?

01 January 1970 6,653 1 View

How i can separate bacterial cells from LB broth to further inoculate it in MSM media ?

01 January 1970 191 4 View

Radical Distribution Function using AMBER

I am trying to run a Radial Distribution Function (RDF) trajectory analysis between water (WAT) hydrogen atoms and ligand (UNN) nitrogen atoms using the production trajectory (prod1.crd) and the...

01 January 1970 7,434 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View