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If I am considering Taq polymerase to PCR amplifying 1.3kb of 7.5kb genome, knowing that the mutation rate of Taq is 1 base in 10000 bases (it can be translated in this context as 1 base every ~8...
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I have transfected L929 cells with polio virus in T-75 flask. I left it for 48 hours and now I collected the media which is 15ml DMEM. I want the best way to isolate the viral RNA from this media....
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