Has anyone experience doing tissue culture and having two types of tips in culture? One having a terminal shoot tip and Z-like stem and many roots, and the other with a terminal leaf, sub-apical tip, straight stem and no roots?
Beleives of childrens learning in school
26 May 2024 1,303 3 View
I am trying to model a variable thickness composite part in LS-DYNA and I am unsure on how to implement this. I have used PART_COMPOSITE in the past when testing with a composite part with the...
23 April 2024 9,376 1 View
m=8 for i=1:m-1 for b=1:m for c=1:m F(b)=[E(1),E(2),E(3),E(4),E(5),E(6),E(7),E(8)]* matrix(:,i+1) - t_val *[E(1),E(2),E(3),E(4),E(5),E(6),E(7),E(8)]* matrix1* matrix(:,i+1) - delta...
22 February 2024 9,005 1 View
I am planning to isolate EVs from skeletal muscle samples and see that most studies doing so incubate fresh tissue in media for EV isolation. I am wondering what the drawbacks of using flash...
30 January 2024 7,979 0 View
Issue in addressing police research study gaps in literature to identify job satisfaction or dissatisfaction among police officers. Historical literature on police officer job satisfaction not an...
05 October 2023 5,290 0 View
How do I add supplemental material from a published OA paper ?
20 September 2023 6,335 2 View
Hi all, Does anyone know of a kit for measuring HMG coA synthase in cell lysates? I will prefer a non-radioactive kit that can be used with a spectrophotometer. Thanks in advance. Sylvia
30 July 2023 267 3 View
A friend of mine majors in math. He is confused about the symbol ℘ appearing in Complex Analysis written by Elias M. Stein and Rami Shakarchi. He tells me that it can be considered as "P" in some...
07 July 2023 2,883 1 View
I am using my smartphone to record the frog calls overnight, How can i tell which species is more dominant from the calls they make?
21 June 2023 8,703 1 View
Hey, I am currently writing my Master thesis and I'm conducting an fsQCA using the QCA package in R Studio. In total, I have 9 causal conditions which I all calibrated already. However, I found...
24 May 2023 9,255 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View