Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Are there any simple materials besides activated carbon that can remove a variety of dissolved organic chemicals in water samples?

I am looking for a method to remove a suite of dissolved organic compounds (e.g. BTEX, PAHs, PCBs, acetone/acetate/acetic acid, phthalates, phenols, glycols, biocides, surfactants...) that are...

12 February 2020 9,656 3 View

Is there a way to simultaneously visualize DNA and protein following electrophoresis?

I am currently optimizing the conjugation of a DNA oligo sequence to a DBCO activated antibody and am looking for a method which could allow me to simultaneously visualize the amount of DNA which...

15 May 2019 9,228 3 View

Can anyone point me to literature exploring WHY researchers study what they do?

I'm looking at this issue in relation to education research in Africa, but very interested in insights from other fields and contexts, too.

04 September 2018 6,158 8 View

Log or square root transformation?

Hello, I recently analysed count data (in this case the no of stems per tree) using a GLM. The analysis was carried out on both log transformed and square root transformed data. Although...

10 February 2017 8,300 7 View

Does anyone have experience with methods to overcome fluorescent overlap between Doxorubicin and mCherry?

I am looking to assess the metabolic control of breast epithelial cancer cells in cancer-associated fibroblasts (CAFs) and the subsequent effect of this on doxorubicin chemoresistance using an in...

22 January 2017 2,175 1 View

The answer to TPN hyperglycemia control.

11 December 2016 6,168 6 View

Best Method for Quantifying miRNA in Sperm Samples?

We have collected sperm samples and wish to measure miRNA content. What is the best method/kits to go with from extraction of RNA all the way to PCR?

15 November 2016 3,488 2 View

Meaning making of having a genetic condition?

31 March 2016 3,442 4 View

Can anyone help me with my a stent and balloon inflation model in Abaqus?

22 November 2015 6,911 15 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View