Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Are there any simple materials besides activated carbon that can remove a variety of dissolved organic chemicals in water samples?

I am looking for a method to remove a suite of dissolved organic compounds (e.g. BTEX, PAHs, PCBs, acetone/acetate/acetic acid, phthalates, phenols, glycols, biocides, surfactants...) that are...

12 February 2020 9,656 3 View

Is there a way to simultaneously visualize DNA and protein following electrophoresis?

I am currently optimizing the conjugation of a DNA oligo sequence to a DBCO activated antibody and am looking for a method which could allow me to simultaneously visualize the amount of DNA which...

15 May 2019 9,228 3 View

Can anyone point me to literature exploring WHY researchers study what they do?

I'm looking at this issue in relation to education research in Africa, but very interested in insights from other fields and contexts, too.

04 September 2018 6,158 8 View

Log or square root transformation?

Hello, I recently analysed count data (in this case the no of stems per tree) using a GLM. The analysis was carried out on both log transformed and square root transformed data. Although...

10 February 2017 8,300 7 View

Does anyone have experience with methods to overcome fluorescent overlap between Doxorubicin and mCherry?

I am looking to assess the metabolic control of breast epithelial cancer cells in cancer-associated fibroblasts (CAFs) and the subsequent effect of this on doxorubicin chemoresistance using an in...

22 January 2017 2,175 1 View

The answer to TPN hyperglycemia control.

11 December 2016 6,168 6 View

Best Method for Quantifying miRNA in Sperm Samples?

We have collected sperm samples and wish to measure miRNA content. What is the best method/kits to go with from extraction of RNA all the way to PCR?

15 November 2016 3,488 2 View

Meaning making of having a genetic condition?

31 March 2016 3,442 4 View

Can anyone help me with my a stent and balloon inflation model in Abaqus?

22 November 2015 6,911 15 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

Best analysis method to use for my variables?

I need to conduct an analysis and wanted to check if my idea for analysis sounds feasible. I have 3 DVs which are; fear of terrorism, confidence in the police and punitive attitudes towards...

07 February 2021 5,746 10 View

Question regarding appropriate statistical analysis?

We have a data set from N=193 participants. They assessed Corporate Image, Employer Image, Customer Experience and Employer Attractiveness (on Likert scales) for 8 different companies (each of...

30 January 2021 3,296 4 View

Are we really behind certifications for getting a better job or it is somewhere related to our self satisfaction or learning new things?

Certificate, Job, Self Satisfaction

26 January 2021 487 13 View

Is it possible to combine two continuous variables into one categorical variable in order to create a dummy in SPSS ?

As background, in my model, my independent variable is visual clutter which has two conditions (higher visual clutter/ low visual clutter) and the conditions measure the amount of visual clutter...

13 January 2021 4,760 4 View

What is the current status of black female police officers?

I am a doctoral candidate at Walden University. My dissertation is centered on the lived experience of black female police officers and their perspective of excessive use of force administered...

29 December 2020 8,452 1 View

Recommended models for predicting student satisfaction?

I teach in higher education and obviously aim to create an educational experience my students will find satisfying. Can you recommend literature or predictor models of undergraduate course student...

13 December 2020 6,869 4 View

Will automated killing diminish the error rate by police?

10 December 2020 5,548 3 View

How to calculate sample size?

The current study Digital Marketing in Indian and its Impact on Consumer behavior in post and Pre-covid-19 in Agra a district of the state utttar pradesh India has to be taken up . How should I go...

06 December 2020 5,614 4 View

The relationship between religious adherence and indicators of wellbeing (happiness, satisfaction with life, etc.)?

I am interested in research results concerning the relationship between religious adherence and indicators of wellbeing (happiness, satisfaction with life, etc.).

16 November 2020 3,869 3 View