Sandy Cole

4 Questions 4 Answers 0 Followers

Questions related from Sandy Cole

What kind of Na azide for Flow Cytometry experiment?

I need to use NaN3 as a blocking reagent to prevent cells from capping and internalizing. What strength do I buy it at and what concentration should I use it at? Does anyone have a protocol for...

02 February 2020 1,946 2 View

Has anyone measured IL2R by Flow Cytometry?

I have a request to measure IL2R on different cell lines by Flow Cytometry. Is this usually on the surface?

11 November 2019 6,001 3 View

Are there any 96-well plates or 5 mL FACS tubes that monos will not stick to?

We are trying to develop a Flow Cytometry assay and need to incubate overnight, using untreated monos and LPS-stimmed monos. Our LPS is just for development purposes, but we would like to find a...

10 October 2019 2,922 4 View

Can Live/Dead be added to surface staining cocktail?

I want to add the Live/Dead to my surface staining cocktail and stain once so I don't lose cells with extra washes, i.e. Live/Dead>wash>surface stain>wash again.

11 November 2018 5,164 3 View