We are trying to develop a Flow Cytometry assay and need to incubate overnight, using untreated monos and LPS-stimmed monos. Our LPS is just for development purposes, but we would like to find a vessel our monos won't stick to. The end goal is to treat monos with and without drug to see if the drug is inadvertantly stimming the monos.
Hey Sandy,
we had the same problem with our peritoneal macrophages. We used non-treated 96-Well plates (COSTAR Cat# 3370) and at the time point of FACS analysis we reomved the medium and treated the macrophages with PBS + 2mM EDTA for 7 minutes at 37 °C. Then they can be detached by slowly pipetting up and down 5-10 times. The suspension then went directly into the FACS tubes for analysis.
I hope that helps you.
All the best and a nice weekend,
Marc
try polypropylene tubes. monocytes will adhere to polystyrene or tissue-culture treated plates since they are charged.
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs