What kind of Na azide for Flow Cytometry experiment?

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Has anyone measured IL2R by Flow Cytometry?

I have a request to measure IL2R on different cell lines by Flow Cytometry. Is this usually on the surface?

10 November 2019 5,927 3 View

Are there any 96-well plates or 5 mL FACS tubes that monos will not stick to?

We are trying to develop a Flow Cytometry assay and need to incubate overnight, using untreated monos and LPS-stimmed monos. Our LPS is just for development purposes, but we would like to find a...

09 October 2019 2,864 4 View

Can Live/Dead be added to surface staining cocktail?

I want to add the Live/Dead to my surface staining cocktail and stain once so I don't lose cells with extra washes, i.e. Live/Dead>wash>surface stain>wash again.

10 November 2018 5,004 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Anybody write me the name of scopus journals in commerce, management, economics areas. Which publish without any charge?

Please write those scopus journal names for which you have good experience with them.

24 February 2021 6,210 1 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

What are effects of steel jacketing on flexural strength?

Dear all, If steel jacketing is used to retrofit a reinforced-concrete (RC) column, can we use it to improve flexural strength? I understand that we leave gaps between the ends of the jacket and...

22 February 2021 1,906 5 View