Sara Parker

5 Questions 16 Answers 0 Followers

Questions related from Sara Parker

Phenotype or Artifact in Mouse Brain Slices?

Hi! I am looking at cortical neurons in coronal sections from mice expressing Thy-YFP and a top secret genetic manipulation :) I noticed these vacuoles in the apical dendrites of most of the...

02 February 2019 7,049 7 View

Short CMV Promoter (~350bp): What is this called?

Hi! Some of the lentiviral transfer plasmids I'm using have this short form of the CMV promoter that is 350bp instead of 589bp for the usual CMV promoter. Does this short form have a name?...

11 November 2018 5,111 2 View

Identifying glia subpopulations by IF

Hey Gang, Very easy question to someone who knows more about identifying glia than I do! I am using antibodies against S100 to mark astrocytes and Iba1 to mark microglia in a culture of primary...

06 June 2017 705 2 View

Can anyone recommend a great His-tag antibody for IP?

Thought I'd ask the experts before buying. Thanks in advance!

04 April 2017 6,570 1 View

Multisite Gateway Problem - Mosaic Expression?

Hello ResearchGate Community! I'm building polycistronic lentiviral vectors for the expression of multiple fluorescently-tagged proteins using Multisite Gateway, and I'm finding that I get mosaic...

11 November 2016 6,046 2 View