Dear All,
I am plainning to use hydrogen peroxide to treat to cell and see its protein signaling response.
Most of reference articles use Sigma's hydrogen peroxide solution. Unfortunately, Sigma's hydrogen peroxide cannot be imported to Thailand. I found that Merck's hydrogen peroxide solution is available in Thailand. But I couldn't find much references about using it to cell experiment and it doesn't mention the product number. On the Merck website, the product are not specified grade clearly, (eg. ACS or cell biology) and no reference about it.
If you have used Merck's H2O2 with cell treatment experiment, Could you kindly share which product number is?
Thank you
Cheerio,
H2O2 usually does not come in pure form (for safety reasons).
You can ask a chemist friend to help titrate and determine the effective stock concentration for you.
Have never used it. The issue is whether other components in the solution have biological effect or not. If it is not biological grade then it may contain minimal amount of heavy metals which maybe toxic to the cells or produce unexpected results. Even smallest amount of heavy metals in water can give you some free radical formation in the solution.
I have Used merck. 107210 Hydrogen peroxide 30% to stress human cells and it worked well. Right know i´m looking for for smaller presentation (like vials for injectable treatments). best regards
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