I'd like to investigate EpCAM and MUC1 expression in MCF7 and MDAMB231 cell lines by flow cytometry. Which brand of antibody do you recommend?

In addition, I'd like to investigate cytokeratin expression in same breast cancer cell lines but I can't decide to use which cytkokeratin?

17 October 2020 5,794 6 View

Is it suitable to use a density-based solver to solve supercritical CO2 laminar flow?

We have been numerically investigating the thermal-fluid characteristic of sCO2 laminar flowing in a 0.5 mm diameter circular microtube in upward direction. Depending on sharp density change along...

29 June 2020 5,199 2 View

How can I calculate the frequency of specific words for each row in the dataframe with R?

Hi, I'm working on a data that each sentence is in separate rows in dataframe. I want to determine word frequency in each row with a word list that I have created. Then I would like to add these...

28 October 2018 5,699 10 View

Is there any way to get tweets in a particular time frame from Twitter without using any hashtags or words via Twitter API?

Hello to everyone. Tweets can be downloaded through the R with the Twitter API. Various filters can be used for these tweets (e.g. language, region, time period). While doing so, a word or...

12 October 2018 7,200 3 View

What is your pump manufacturer recommendations, ensuring low flow rate and high operating pressure?

We have recently designed an experimental setup to investigate the detailed thermofluidic mechanism of near-critical CO2. We need to pump, ensuring low flow rate, high operating pressure and also...

22 July 2018 3,359 3 View

I will have alginate polymer films ultramicrotomed to get 100 nm thick slices. Do I need cryo microtomy, what liquid should the specimens be cut onto?

My samples dissolve in water. Dry sectioning does not work on them. Therefore, I need a different liquid to section them onto. I do not know if ethanol or any other liquid would be helpful. Also,...

16 December 2017 4,896 5 View

What extent reliable are studies done in concrete technology without their segregation stability information?

13 July 2017 4,803 5 View

Which software is better about modeling of self-consolidating concrete to simulate its segregation behaviour

There are some commercial softwares to model the SCC in fresh state in point of segregation with coupled restrainments (aggregate and reinforcing bar) . e.g. Flow3D, Fluent, etc. What are...

29 May 2017 8,476 3 View

What is the precautions to avoid the negative effects of hydrogen sulphide on heat exchanger copper fin?

23 August 2015 2,445 3 View

How can I calculate enthalpy uncertainty value which is necessary for laboratory refrigerant side capacity?

27 April 2015 8,148 8 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View