You can dilute your LPS stock solution to 2 µg/µL using a simple mathematical formula and careful pipetting techniques. Here's what you'll need:
Your original LPS stock solution (concentration known in µg/mL)
Sterile, endotoxin-free water (important to avoid contaminating your LPS)
1. Calculate the dilution factor:
The dilution factor is the ratio of the final desired concentration (2 µg/µL) to the concentration of your original stock solution (X µg/mL).
Denoting the dilution factor as "D":
D = Final concentration / Original concentration D = 2 µg/µL / X µg/mL
2. Determine the volume of stock solution to use:
Once you have the dilution factor (D), you can calculate the volume (V₁) of your original stock solution needed to prepare 1 µL of your 2 µg/µL final solution.
V₁ = 1 µL (final volume) / D
3. Determine the volume of diluent to add:
The volume (V₂) of sterile water you need to add to reach your final volume of 1 µL is simply: