It depends on the concentration of your stock. Once you know that, you simply have to solve Cstock * Vstock = [2µg/µl] * Vfinal

There are several online calculators that can help too (e.g., https://www.tocris.com/resources/dilution-calculator)

Hi Emine Gökce

You can dilute your LPS stock solution to 2 µg/µL using a simple mathematical formula and careful pipetting techniques. Here's what you'll need:

• Your original LPS stock solution (concentration known in µg/mL)
• Sterile, endotoxin-free water (important to avoid contaminating your LPS)

1. Calculate the dilution factor:

The dilution factor is the ratio of the final desired concentration (2 µg/µL) to the concentration of your original stock solution (X µg/mL).

Denoting the dilution factor as "D":

D = Final concentration / Original concentration D = 2 µg/µL / X µg/mL

2. Determine the volume of stock solution to use:

Once you have the dilution factor (D), you can calculate the volume (V₁) of your original stock solution needed to prepare 1 µL of your 2 µg/µL final solution.

V₁ = 1 µL (final volume) / D

3. Determine the volume of diluent to add:

The volume (V₂) of sterile water you need to add to reach your final volume of 1 µL is simply:

V₂ = 1 µL (final volume) - V₁ (volume of stock solution)

4. Prepare your dilution:

• Using sterile technique, pipette the calculated volume (V₁) of your original LPS stock solution into a sterile microcentrifuge tube.
• Then, pipette the calculated volume (V₂) of sterile, endotoxin-free water into the same tube.
• Gently mix the solution by pipetting up and down a few times. Avoid creating bubbles.

You can also use Graph Pad Calculator to do it in seconds.

Hope it helps,

Thanks,

Hi Emine Gökce,

You may use the web tool to calculate

https://www.alfa-chemistry.com/solution-dilution-calculator.htm

https://www.alfa-chemistry.com/mass-molarity-calculator.htm