Got High concentration of DNA 143ug/ml from the soil but the quality is low 1.2. so How to increase the quality of DNA?

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Which primers sets(Sense two sets) Antisense(Two sets) I should use in conventional PCR? Two sense and two antisense primers what does it means?

Dear Researchers, I have read an article and there are two set of primer pairs. Sense primers and antisense primers. The paragraph from the article is as [ The partial DNA-dependent RNA...

31 December 2019 7,702 7 View

Can anyone help in SOD activity buffers?

In 3ml, how can I make all these buffers, I have read this paper for SOD activity, Please find below [The 3 ml reaction mixture contained 50 mM potassium phosphate buffer (pH 7.0), 0.5 mM...

06 July 2019 3,836 6 View

Is there any easy method to get Jasmonic acids from wheat plants?

Dear All, I need to check the growth promoting effects of some bacterial antagonists. I want to obtain the jasmonic acid and other growth promoting hormones. Then I want to do LCMS. Any step by...

05 June 2019 2,710 4 View

How to prepare solution for GC-MS/LC-MS/HPLC from the barley root exudates/leaf extracts?

I want to check some metabolites from the root exudates or leaf extracts. Than I want to check the metabolites concentration like Jasmonic acid, via GC-MS, LC-MS or HPLC. Can anyone explain me...

03 April 2019 3,524 5 View

Can anyone explain PERMANOVA pairwise results?

Dear All, Can anyone explain the pairwise permanova for the group 1 and Group 2. The data is given as below: Group 1 Group 2 Sample size Permutations pseudo-F p-value q-value A C 12 999...

11 December 2018 5,516 0 View

How can I count conidia on the PDA petriplates incubated for two weeks with haemocytomer?

I have a haemocytometer with 25 major grids and each grid is divided into 16 small grids, The dept is 0.1mm. I put 10 ml ddH20 in petriplates and rub to get spores. I take 10ul from that 10 ml...

01 February 2018 2,835 1 View

What is the definition of the strains in terms of fungi?

Dear All, I have read several papers like these: twelve strains of fungus Trichoderma harzianum were evaluated against Phytopathogen. So in these case what would be the definition of strains?

10 November 2017 6,435 10 View

Can someone recommend a proper procedure for extractions of antibiotics and peptides from the fungi?

Dear All, I want to extract antibiotics and peptides from a fungi. Is there any proper procedure to extract antibiotics from the fungi.

10 November 2017 6,815 3 View

How can I measure the percent inhibition of a plant pathogen caused by bi-control fungus?

Dear All, I have cultured two fungi one 9cm petri plates. The growth of biocontrol agent is very slow but it has potential to parasitize a plant pathogen. I want to measure the percent inhibition...

10 November 2017 5,113 3 View

Could not get a good and clear band in the Gell electrophoresis?

I run the Gell for DNA of some of samples. However some DNA bands were thinner some were more thicker in size and some of them were like smear from top to bottom. What could be the reason?

10 November 2017 9,962 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Paul Rutland

is this animal or plant/soil microbe DNA Abbas? The impurities are different between the types but if human/animal I would proteinase K it and ethanol precipitate or re extract with phenol/chloroform

ALEX CHUN CHEUNG

You may use Phenol / Chloroform for DNA Purification.

here is the protocol.

https://www.thermofisher.com/hk/en/home/references/protocols/nucleic-acid-purification-and-analysis/dna-extraction-protocols/phenol-chloroform-extraction.html

Ajonok Hano

DNA from the Soil,

I don't use kits, I want to sue reagents