I use an extraction buffer to get DNA, I got but the quality is very low. I measure the quality and quantity of DNA using nanodrop spectrophotometer??
is this animal or plant/soil microbe DNA Abbas? The impurities are different between the types but if human/animal I would proteinase K it and ethanol precipitate or re extract with phenol/chloroform
You may use Phenol / Chloroform for DNA Purification.
here is the protocol.
https://www.thermofisher.com/hk/en/home/references/protocols/nucleic-acid-purification-and-analysis/dna-extraction-protocols/phenol-chloroform-extraction.html
DNA from the Soil,
I don't use kits, I want to sue reagents
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