What's the best commercially available microbe gDNA extraction kit?

Could anyone suggest the best commercially available microbe gDNA extraction kit ? We would like to extract the microbe gDNA from mouse lung tissues and stool samples for downstream microbiome...

08 September 2018 5,848 4 View

How can I plot Box-whisker using the data points exported from REST 2009?

10 November 2015 7,709 1 View

Can real-time PCR be used to assess the expression of mutated RNA in controls vs patients?

I have patients and controls who share exactly the same heterozygous mutation in one of their protein. Controls are obviously healthy- So my plan is to see which allele is prominent when it come...

08 September 2015 8,891 7 View

What sort of software can be used to generate a figure like this?

I'm interested in generating a figure similar to A in the attached image. Can anyone suggest a software which can be used to generate such. Thanks Harindra

03 April 2015 9,878 7 View

How do yo determine which small RNA or RNAs are acting on a particular gene?

I'd like to see if either microRNA or siRNA affects the translation of my gene of interest (protein). I understand that there are kits which detect small RNAs from total RNAs but how can I...

11 December 2014 339 7 View

What is the best free alternative to Beacon Designer?

Beacon designer is quite a sophisticated primer and probe designing tool which is available commercially. Anyone to suggest me a free alternative that can do almost the same or closer? Thanks

10 November 2014 9,909 5 View

Where to upload my sequence reads from a study?

I have recently done an analysis to detect genomic alternations in patients and healthy controls. Entire coding region from the gene of interest was sequenced using bi-directional sanger...

10 November 2014 2,979 1 View

What determines the most suitable substitution model in BEAST?

Can somebody explain to me how exactly to chose the right substitution model in BEAST? I understand there are some modelling softwares that suggest the most appropriate model. But what in general...

08 September 2014 9,080 3 View

How can I visualise the structures of HLA alleles?

I have a couple of peptides (epitopes) with corresponding HLA alleles. Any possibility that I can visualise the structure of HLA alleles along with the bound epitope and to see how the epitope...

08 September 2014 1,586 9 View

What is the best safety glasses to use within a bio lab?

Anyone to suggest a good safety glass brand or a model to use in a biological lab. I found couples of UVEX glasses, but I'm not sure they're appropriate to use in a bio lab. They actually do...

07 August 2014 719 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View