What's the best commercially available microbe gDNA extraction kit?

Could anyone suggest the best commercially available microbe gDNA extraction kit ? We would like to extract the microbe gDNA from mouse lung tissues and stool samples for downstream microbiome...

08 September 2018 5,848 4 View

How can I plot Box-whisker using the data points exported from REST 2009?

10 November 2015 7,709 1 View

Can real-time PCR be used to assess the expression of mutated RNA in controls vs patients?

I have patients and controls who share exactly the same heterozygous mutation in one of their protein. Controls are obviously healthy- So my plan is to see which allele is prominent when it come...

08 September 2015 8,891 7 View

What sort of software can be used to generate a figure like this?

I'm interested in generating a figure similar to A in the attached image. Can anyone suggest a software which can be used to generate such. Thanks Harindra

03 April 2015 9,878 7 View

How do yo determine which small RNA or RNAs are acting on a particular gene?

I'd like to see if either microRNA or siRNA affects the translation of my gene of interest (protein). I understand that there are kits which detect small RNAs from total RNAs but how can I...

11 December 2014 339 7 View

What is the best free alternative to Beacon Designer?

Beacon designer is quite a sophisticated primer and probe designing tool which is available commercially. Anyone to suggest me a free alternative that can do almost the same or closer? Thanks

10 November 2014 9,909 5 View

Where to upload my sequence reads from a study?

I have recently done an analysis to detect genomic alternations in patients and healthy controls. Entire coding region from the gene of interest was sequenced using bi-directional sanger...

10 November 2014 2,979 1 View

What determines the most suitable substitution model in BEAST?

Can somebody explain to me how exactly to chose the right substitution model in BEAST? I understand there are some modelling softwares that suggest the most appropriate model. But what in general...

08 September 2014 9,080 3 View

How can I visualise the structures of HLA alleles?

I have a couple of peptides (epitopes) with corresponding HLA alleles. Any possibility that I can visualise the structure of HLA alleles along with the bound epitope and to see how the epitope...

08 September 2014 1,586 9 View

No title

06 July 2014 7,928 12 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View