What's the best commercially available microbe gDNA extraction kit?

Could anyone suggest the best commercially available microbe gDNA extraction kit ? We would like to extract the microbe gDNA from mouse lung tissues and stool samples for downstream microbiome...

08 September 2018 5,848 4 View

How can I plot Box-whisker using the data points exported from REST 2009?

10 November 2015 7,709 1 View

Can real-time PCR be used to assess the expression of mutated RNA in controls vs patients?

I have patients and controls who share exactly the same heterozygous mutation in one of their protein. Controls are obviously healthy- So my plan is to see which allele is prominent when it come...

08 September 2015 8,891 7 View

How do yo determine which small RNA or RNAs are acting on a particular gene?

I'd like to see if either microRNA or siRNA affects the translation of my gene of interest (protein). I understand that there are kits which detect small RNAs from total RNAs but how can I...

11 December 2014 339 7 View

What is the best free alternative to Beacon Designer?

Beacon designer is quite a sophisticated primer and probe designing tool which is available commercially. Anyone to suggest me a free alternative that can do almost the same or closer? Thanks

10 November 2014 9,909 5 View

Where to upload my sequence reads from a study?

I have recently done an analysis to detect genomic alternations in patients and healthy controls. Entire coding region from the gene of interest was sequenced using bi-directional sanger...

10 November 2014 2,979 1 View

What determines the most suitable substitution model in BEAST?

Can somebody explain to me how exactly to chose the right substitution model in BEAST? I understand there are some modelling softwares that suggest the most appropriate model. But what in general...

08 September 2014 9,080 3 View

How can I visualise the structures of HLA alleles?

I have a couple of peptides (epitopes) with corresponding HLA alleles. Any possibility that I can visualise the structure of HLA alleles along with the bound epitope and to see how the epitope...

08 September 2014 1,586 9 View

What is the best safety glasses to use within a bio lab?

Anyone to suggest a good safety glass brand or a model to use in a biological lab. I found couples of UVEX glasses, but I'm not sure they're appropriate to use in a bio lab. They actually do...

07 August 2014 719 2 View

No title

06 July 2014 7,928 12 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View