What's the best commercially available microbe gDNA extraction kit?

Could anyone suggest the best commercially available microbe gDNA extraction kit ? We would like to extract the microbe gDNA from mouse lung tissues and stool samples for downstream microbiome...

08 September 2018 5,848 4 View

How can I plot Box-whisker using the data points exported from REST 2009?

10 November 2015 7,709 1 View

Can real-time PCR be used to assess the expression of mutated RNA in controls vs patients?

I have patients and controls who share exactly the same heterozygous mutation in one of their protein. Controls are obviously healthy- So my plan is to see which allele is prominent when it come...

08 September 2015 8,891 7 View

What sort of software can be used to generate a figure like this?

I'm interested in generating a figure similar to A in the attached image. Can anyone suggest a software which can be used to generate such. Thanks Harindra

03 April 2015 9,878 7 View

How do yo determine which small RNA or RNAs are acting on a particular gene?

I'd like to see if either microRNA or siRNA affects the translation of my gene of interest (protein). I understand that there are kits which detect small RNAs from total RNAs but how can I...

11 December 2014 339 7 View

Where to upload my sequence reads from a study?

I have recently done an analysis to detect genomic alternations in patients and healthy controls. Entire coding region from the gene of interest was sequenced using bi-directional sanger...

10 November 2014 2,979 1 View

What determines the most suitable substitution model in BEAST?

Can somebody explain to me how exactly to chose the right substitution model in BEAST? I understand there are some modelling softwares that suggest the most appropriate model. But what in general...

08 September 2014 9,080 3 View

How can I visualise the structures of HLA alleles?

I have a couple of peptides (epitopes) with corresponding HLA alleles. Any possibility that I can visualise the structure of HLA alleles along with the bound epitope and to see how the epitope...

08 September 2014 1,586 9 View

What is the best safety glasses to use within a bio lab?

Anyone to suggest a good safety glass brand or a model to use in a biological lab. I found couples of UVEX glasses, but I'm not sure they're appropriate to use in a bio lab. They actually do...

07 August 2014 719 2 View

No title

06 July 2014 7,928 12 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View