Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Prediction of novel precursor miRNAs in viral genomes?

Dear, I performed a multi-sequence alignment (MSA) with all the complete genome sequences of SARS-related viruses. I then used RNALalifold from the ViennaRNA package, with default parameters, to...

06 December 2020 1,704 3 View

How to extract within-group effects from a mixed effects model in R?

Hello, I am working with some longitudinal data resulted from a clinical trial. I have the interaction effect of time and group + random effect of Subject. Time variable consists of 6 timepoint...

29 November 2020 6,416 3 View

How can I apply degenerate primer in SerialCloner (2.6.1) to run a PCR?

I have to carry out an in silico PCR on the 16S-rRNA gene of 5 random bacteria strains. I would like to evaluate the resulting amplicon of different primer pairs. Because I can only use free...

14 November 2020 2,341 2 View

Random effect: include or exclude, considering ICC?

We conducted a field trail, where a harvesting machine repeatedly passed numerous study plots (randomly located in a forest stand). After each pass, rut depth increment was measured. Number of...

08 November 2020 4,232 3 View

Can I calculate mean and confidence intervals of d-prime values?

Hi everybody. I ran an experiment on memory and calculated the percentage of hits as usually reported in the literature. However, the reviewer asked me to transform my hits into d-prime values. I...

21 October 2020 3,859 1 View

Discrepancy between RNA and protein levels in single-cell seq? CD3 mRNA vs protein?

Hi all, A very quick question. I have sorted human T cells from blood using anti-CD3E, CD4 and CD8a antibodies. We did single cell seq on those sorted cells. Analysing the results, I do not see...

19 September 2020 4,953 3 View

How to do a meta analysis--defining a model?

Hi, I wanted to know the fundamental basics of a meta analysis. I have read up and found many diff methods being applied to answer a given research question. I want to understand the below: 1....

20 July 2020 4,445 5 View

What are the nuclei mean number from freeze tissue nuclei isolation protocol?

Hello, everyone! I'm going to start a project with single-cell ATAC-seq for analysis of open chromatin regions from freeze tissue samples. I'm lost about the nuclei concentration. If I made a...

19 June 2020 7,348 2 View

How do I compute for the Pr(Y=1|X=1) H1 and Pr(Y=1|X1) H0 for a priori estimation of required sample size?

I am trying to estimate the required sample size replicate a study which used a probit regression analysis. The paper reported the probit regression results and the marginal effects but I can't...

17 May 2020 4,826 1 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Has anyone experienced a double band GAPDH in Western Blot?

I am using my GAPDH as a housekeeping protein for my Western Blot. For all the blots I have, my GAPDH was coming out as two bands. Therefore, I am using both bands for my analysis. Has anyone...

11 February 2021 6,376 3 View

No signal/bands observed on western blot for ERK signaling?

My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...

30 January 2021 1,039 3 View

Has anyone used Isolate II RNA mini kit for isolation miRNA from exosomes?

I will be isolating exosomes from plasma with Total Exosome Isolation Kit (Invitrogen). Will Isolate II RNA mini kit (Bioline) be appropriate for miRNA isolation from obtained exosomes? The...

26 November 2020 9,503 5 View

Which ABs concentration for EVs immuno-staining?

Hi all, I have to do a phenotyping study on cell culture derived EVs, in particular I should stain them using primary labelled ABs anti-EVs markers before proceeding to analyze them through...

25 November 2020 4,601 1 View

What is the best RNA isolation method from exosomes of serum samples ?

12 November 2020 9,331 7 View

Exosome quantification by Elisa based method ?

Hi, I'm working on exosomes isolated from blood samples and cell culture supernatant. I'm wondering if I could quantify exosomes based on Elisa Method coated with exo Ab (CD63 / CD9/C81...) My...

11 November 2020 664 3 View

How to properly collect/store exosomes (isolated from conditioned culture media) for a later proteomic and/or metabolomic profiling?

I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap...

06 November 2020 5,201 3 View

Could anyone recommend a kit to isolate exosome from cell culture supernatant?

Hello, I'm working with immortalized HUVECs low and I'm trying to study their exosomal RNA contents. Could anyone recommend an effective kit to isolate exosomes from cell culture supernatant?...

31 October 2020 4,252 5 View

Any idea why my Western Blot Stripping buffer doesn't work?

I activated my membrane twice in total and hybridized it with two different antibodies. The first antibody was the specific antibody I was looking for and the second one was GAPDH. I then...

29 October 2020 5,530 6 View