Has anyone experienced a double band GAPDH in Western Blot?

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Mateo Glumac

I never got 2 bands for GAPDH. Can you write which antibody did you use for the blot? Mine is from Cell Signaling Technology. Also what cell line is this?

Racha Al Tannir

Sigma anti-GAPDH rabbit. I am using rat brain. I take my region of interest only and not the entire brain.

Ying Wang

I use anti-GAPDH from Cell Signaling and never have the problem.