I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap freezing in liquid nitrogen then store in -80 ??? or just store in -80 (without snap freezing)?? or are there any specific precautions that should be taken in consideration while sample collection and storage?? Many thanks in advance for sharing your experience.
You may refer following articles for your query
Article Harmonization of exosome isolation from culture supernatants...
Article Harmonization of exosome isolation from culture supernatants...
doi:10.3390/molecules24193516
Many thanks for sharing this article Awanish Kumar . It is really helpful ..
Hello,
In my personal experience, is not a good idea to freeze them, just to avoid rupture. For the isolation, I recommend this manuscript and the corresponding isolation procedure:
PMID: 31434009, DOI: 10.1016/j.jprot.2019.103490
Good luck!
Carlos J.
- Badges
- Science topic
- Similar topics
- Chemistry
- General Biochemistry
- Proteomics