How to extract within-group effects from a mixed effects model in R?

Hello, I am working with some longitudinal data resulted from a clinical trial. I have the interaction effect of time and group + random effect of Subject. Time variable consists of 6 timepoint...

29 November 2020 6,416 3 View

Random effect: include or exclude, considering ICC?

We conducted a field trail, where a harvesting machine repeatedly passed numerous study plots (randomly located in a forest stand). After each pass, rut depth increment was measured. Number of...

08 November 2020 4,232 3 View

How to do a meta analysis--defining a model?

Hi, I wanted to know the fundamental basics of a meta analysis. I have read up and found many diff methods being applied to answer a given research question. I want to understand the below: 1....

20 July 2020 4,445 5 View

Abaqus Error: The surface of a 3D discrete rigid part does not exist in the assembly?

I used a discrete rigid part (cuboid) to translate a force into a plate for a bending test. Trying to run the job, there occurs an error that the surface of the cuboid does not exist. In the...

20 January 2020 5,160 4 View

Advanced analysis of summary data of 2 different surveys?

Hi, I am doing a state wise comparison of nutrition related data of 2 different surveys and I am using their state wise estimates. I have raw data for only 1 data set and I understand that I...

20 January 2020 8,530 3 View

Recommendation on research on Bangladesh/South Asian programs on breastfeeding/nutrition <1 year child?

Hi, I am looking for references on nutrition interventions in south asian nations like Bangladesh and Sri Lanka (mostly nutrition sensitive/preventive approach) to reduce growth...

05 January 2020 7,580 8 View

Suitability of Poisson Regression?

Hi, I am looking at dietary diversity (7 food group score data) as a dependent variable and with 2 continous and 2 categorical variables as independent variables.. Is poisson regression okay for...

14 May 2019 6,906 5 View

Interpretation of Dummy Variable ?

Hi, I have done a linear model with 3 IV including female dummy on weight for height Z score.. I need help with understanding the effect of coeff value (positive value) with regards to intercept...

06 November 2018 7,502 3 View

Hello! how to interpret silver nanoparticle UV-VIS spectra?

Hello experts! I just want to ask how to interpret UV-VIS spectra of Ag nanoparticles. I tried to synthesize AgNP using plant extract and optimize its formation. When I tried varying reaction...

14 October 2018 1,579 4 View

Protocol for quantify microRNA expression?

08 January 2018 8,894 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View