5 Questions 2 Answers 0 Followers
Questions related from Marian Cruz
Hi, everyone. I've been culturing hFOB 1.19 for the last two months. And at first after frozen the cells, they grow well and fast, but after the first subculture the cells starting to grow too...
06 April 2021 172 2 View
Recently I started to work with exosomes, and to verify if I really have this vesicles I used western blot for CD63 as a positive control and GRP94 as negative control, I found bands in CD63 but...
04 October 2018 4,568 1 View
I´ve been workin with fungi RNA, Iextracted RNA with trizol method but my RNA is contaminated with DNA, at least my tutor said that. Anybody can help me with some protocol to treat my RNA with...
21 June 2018 9,062 2 View
I want to quantify the expression of specific miRNAs which I extracted from paraffin tissues, but I want to quantify them and verify they expression. I don´t know if Taqman microRNA assay be the...
09 January 2018 9,012 3 View
I did a Western Blot yesterday but, my band doesn't exist! I think my antibody dilution is wrong... I used 1:10,000 for anti-ERG What dilution should I try this time?
27 October 2016 1,294 1 View
