Will annealing temperature 56 for primer whose melting temperture is 61 work?

I am working on detection of rickettsia in ticks, while I use a primer whose annealing temperature in the original paper 48, I always get non specific bands without appearance of the specific one...

04 May 2019 9,115 5 View

I had pcr contamination in master mix if I exposure the master mix to uv for one hour will this affect pcr and can eliminate the contamination?

Master mix consist of dna polymerase taq and other reagents such as nucleotides can it be negatively affected by exposure to the uv yet can eliminate the contamination?

04 May 2019 7,833 7 View

Does the incubation time for elution DNA affect the purity if sample ?

I am using Qiagen blood and tissue kit for extracting of DNA from ticks I elute the DNA after incubation 1minute as manufacturers instructions If I increase the elusion step to more than 1...

03 April 2019 1,302 4 View

Which is better as master mix kit Qiagen top taq mastermix or taq mastermix provided also by Qiagen ?

My work is on detection of pathogen in ticks so I work on small amount of DNA tamplet copies. while I used Qiagen DNA extraction kit form blood and tissue I am still confused with choosing one of...

10 November 2018 9,124 4 View

What are the most common zoonotic diseases transmitted from pet animals in Egypt ?

07 August 2017 8,596 4 View

Any recommendations for some websites give information about updated emerging infectious diseases like Health map or WHO ????

01 February 2017 7,645 13 View

How can i detect cryptospordium parvum oocyst in vegetables and soil and how can i extract its DNA for PCR reaction ?

03 April 2016 8,083 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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02 March 2021 3,060 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

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28 February 2021 9,936 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View