Asmaa Nasr

8 Questions 12 Answers 0 Followers

Questions related from Asmaa Nasr

Will annealing temperature 56 for primer whose melting temperture is 61 work?

I am working on detection of rickettsia in ticks, while I use a primer whose annealing temperature in the original paper 48, I always get non specific bands without appearance of the specific one...

05 May 2019 9,156 5 View

I had pcr contamination in master mix if I exposure the master mix to uv for one hour will this affect pcr and can eliminate the contamination?

Master mix consist of dna polymerase taq and other reagents such as nucleotides can it be negatively affected by exposure to the uv yet can eliminate the contamination?

05 May 2019 7,880 7 View

Does the incubation time for elution DNA affect the purity if sample ?

I am using Qiagen blood and tissue kit for extracting of DNA from ticks I elute the DNA after incubation 1minute as manufacturers instructions If I increase the elusion step to more than 1...

04 April 2019 1,382 4 View

Which is better as master mix kit Qiagen top taq mastermix or taq mastermix provided also by Qiagen ?

My work is on detection of pathogen in ticks so I work on small amount of DNA tamplet copies. while I used Qiagen DNA extraction kit form blood and tissue I am still confused with choosing one of...

11 November 2018 9,167 4 View

Fungal DNA extraction kit?

Does anyone has any idea about the extraction kit should I use to extract DNA of the histoplasma fungi from clincal sample and the fungal culture?

12 December 2017 4,876 4 View

What are the most common zoonotic diseases transmitted from pet animals in Egypt ?

There are many of zoonotic diseases transmitted from pet animals in books and scientific articals, but I need real field experienced persons to provide this information what are the most commonly...

08 August 2017 8,642 4 View

Any recommendations for some websites give information about updated emerging infectious diseases like Health map or WHO ????

I want any suggestions for online websites which contain updated information concerning emerging infectious diseases. Thanks in advance

02 February 2017 7,687 13 View

How can i detect cryptospordium parvum oocyst in vegetables and soil and how can i extract its DNA for PCR reaction ?

Cryptosporidium parvum is water borne pathogen ,water contaminated by oocyst can lead to contamination of vegetable and soil but the concentration of oocyst may be low so what is the best method...

04 April 2016 8,119 3 View