My work is on detection of pathogen in ticks so I work on small amount of DNA tamplet copies. while I used Qiagen DNA extraction kit form blood and tissue I am still confused with choosing one of these kits especially as top taq mastermix kit use a thermostable DNA polymerase from a source other than the standard taq polymerase orginated from thermas aquaticus in the taq mastermix kit
both have the same extension rate and fidelity
Does it really matter the type of the thermostable polmerase in this situation ?
In my knowledge there is no differences for you about those kit you said.
Most different between DNA polymerizes are their power proof reading, extension size and etc, but I do not have heard about copy number.
Any way look at this
Chapter DNA polymerases for PCR applications
and if you find more information specially about copy number in samples, please let me know.
In my opinion, choose one sample as an experiment from the extraction if DNA into the Mastermix you want and check the results.
My experience, hermostable DNA polymerase , gave high sensitivity of result when i test same type of sample from bacteria species. I am not sure your case. I suggest you spend sometime test it.
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