Will annealing temperature 56 for primer whose melting temperture is 61 work?

I am working on detection of rickettsia in ticks, while I use a primer whose annealing temperature in the original paper 48, I always get non specific bands without appearance of the specific one...

04 May 2019 9,115 5 View

I had pcr contamination in master mix if I exposure the master mix to uv for one hour will this affect pcr and can eliminate the contamination?

Master mix consist of dna polymerase taq and other reagents such as nucleotides can it be negatively affected by exposure to the uv yet can eliminate the contamination?

04 May 2019 7,833 7 View

Does the incubation time for elution DNA affect the purity if sample ?

I am using Qiagen blood and tissue kit for extracting of DNA from ticks I elute the DNA after incubation 1minute as manufacturers instructions If I increase the elusion step to more than 1...

03 April 2019 1,302 4 View

Which is better as master mix kit Qiagen top taq mastermix or taq mastermix provided also by Qiagen ?

My work is on detection of pathogen in ticks so I work on small amount of DNA tamplet copies. while I used Qiagen DNA extraction kit form blood and tissue I am still confused with choosing one of...

10 November 2018 9,124 4 View

Fungal DNA extraction kit?

Does anyone has any idea about the extraction kit should I use to extract DNA of the histoplasma fungi from clincal sample and the fungal culture?

11 December 2017 4,829 4 View

What are the most common zoonotic diseases transmitted from pet animals in Egypt ?

07 August 2017 8,596 4 View

How can i detect cryptospordium parvum oocyst in vegetables and soil and how can i extract its DNA for PCR reaction ?

03 April 2016 8,083 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View