Will annealing temperature 56 for primer whose melting temperture is 61 work?

I am working on detection of rickettsia in ticks, while I use a primer whose annealing temperature in the original paper 48, I always get non specific bands without appearance of the specific one...

04 May 2019 9,115 5 View

I had pcr contamination in master mix if I exposure the master mix to uv for one hour will this affect pcr and can eliminate the contamination?

Master mix consist of dna polymerase taq and other reagents such as nucleotides can it be negatively affected by exposure to the uv yet can eliminate the contamination?

04 May 2019 7,833 7 View

Does the incubation time for elution DNA affect the purity if sample ?

I am using Qiagen blood and tissue kit for extracting of DNA from ticks I elute the DNA after incubation 1minute as manufacturers instructions If I increase the elusion step to more than 1...

03 April 2019 1,302 4 View

Which is better as master mix kit Qiagen top taq mastermix or taq mastermix provided also by Qiagen ?

My work is on detection of pathogen in ticks so I work on small amount of DNA tamplet copies. while I used Qiagen DNA extraction kit form blood and tissue I am still confused with choosing one of...

10 November 2018 9,124 4 View

Fungal DNA extraction kit?

Does anyone has any idea about the extraction kit should I use to extract DNA of the histoplasma fungi from clincal sample and the fungal culture?

11 December 2017 4,829 4 View

What are the most common zoonotic diseases transmitted from pet animals in Egypt ?

07 August 2017 8,596 4 View

Any recommendations for some websites give information about updated emerging infectious diseases like Health map or WHO ????

01 February 2017 7,645 13 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View