Freezing BHK cells

Marcio Andrés De Avila Arias @Marcio-De-Avila-Arias

18 October 2016 1 5K Report

there is a protocol cryopreservation of BHK cells in 10% DMSO, and let incubating for 2 hours at 4 °C and then at -80?

I am freezing my cells as follows: 10% DMSO + 90% fetal calf serum then add ampoules at -20 C untill complete freezing then add ampoules at - 70 C overnight then add in liquid nitrogen

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Science method

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