PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

What is the correct way to apply a Design effect for sample size calculation when the sampling method is linear systematic random sampling?

When we are doing sampling by means of simple systematic random sampling,how do we calculate the design effect for use during calculation of sample size for our work?

20 January 2021 5,328 3 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

How to generate .dlg files on PyRx?

Hi. I'm using the PyRx version 0.8 and getting the error message: WARNING: Filename mismatch: "2DFN_ADP_CL.maps.fld" :: "..\2DFN_ADP_CL.maps.fld" C:\Program Files (x86)\The Scripps Research...

17 January 2021 843 3 View

How to convert isotrophic Deriche filter into anistrophic?

12 February 2021 5,889 6 View

Hi all, would you suggest good books for Image processing, Computer vision, AI, Deep learning, machine learning, and remote sensing?

I would like to get good books for Image processing, Computer vision, AI, Deep learning, machine learning, and remote sensing. Kindly suggest good authors books for the above topics and its links...

09 February 2021 3,561 11 View

Does anyone know about vision-guided control of civil structures?

I would like to know more about the following issues: 1. Using images as feedback for the closed-loop control of civil structures 2. Simulating the vision-guided control on the computer without...

03 January 2021 7,566 1 View

Why both Training and Validation accuracies stop improving after some epochs?

I am using data augmentation and decaying learning rate after each epoch. If I don't use data augmentation but keeping callback, then my training accuracy reaches to 99.65% but not validation....

15 December 2020 716 10 View

What are the tools and techniques for making Custom dataset for YOLO?

Dear Research Community. I am new to the field of deep learning based object detection techniques. At this time, I am working on a project which will not use front-facing camera to detect people....

13 December 2020 957 3 View

What is the best machine-learning model for counting overlapping objects?

I am developing a machine-learning model to make accurate and fast counting of metal pipe with difference cross-sectional shapes. Well-define rectangular, triangular and circular shapes are quite...

10 December 2020 2,401 4 View

GAN as Data Augmentation?

Hi, I want to apply GAN as a data-augmentation technique in my model. I am bit confused regarding implementation that how could I give the generated images of GAN to my model on run-time? because...

07 December 2020 4,703 3 View

What is the best Machine learning method to extract collagen bundles features ?

what is the best machine learning method , to segment and extract the length , angle and the size of collagen bundles pictures obtained from the human cornea?

07 December 2020 336 6 View

Does anyone knows any technique to find the location of an object in an image?

We are doing a project in which we are detecting (using YOLOv4) runway debris using a fixed camera mounted at a pole on the side of the runway. We want to find out the position of the object with...

03 December 2020 774 6 View

How to improve accuracy of hard classes in multiclass classification?

Hi, I am training my models to classify 10 classes. Some of the classes are easy for my models and achieve accuracy greater than 95% but some classes have very hard patterns and accuracy stops at...

03 December 2020 324 9 View