Fragment analyzer for estimating miRNAs concentration?

09 September 2017 1 8K Report

Hello,

I am working on exosomes of serum and plasma and tried isolating RNA from exosomes that preserves miRNAs using the miRCURY kit. I tried assessing the sample concentration using the high sensitivity fragment analyzer and have been getting very low concentrations with a very poor quality as well. Can I use any other assay like the Qubit miRNA assay to estimate the concentration of miRNAs in my samples? Any suggestions would be helpful!

Thanks,

Sean Bresnahan

We have used Qubit assay for very small fragments (~20-40bp in length) and have been successful.

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