Hello,
I have exosomal RNAs isolated from serum and plasma specimens of healthy and patient cases whose concentration is very low. The core facility at our university requires at least 1-10 ng to begin with and obtaining that high of an amount from exosomal serum or plasma has been very challenging. We want to perform a high-sensitivity exo-miRNA sequencing that can detect as low as picograms of RNA input. Can someone suggest good sequencing core facilities in the US that can perform this? Any suggestions or inputs would be hugely appreciated.
We worked with Arraystar to sequence microRNA from low quantity RNA samples like milk exosomes. You might want to see if they can help. They usually offer a great bioinformatic analysis with the sequencing they perform. No matter the platform you are working with, ask also about the single cell kits they sometimes use as these can process very little amount of RNA.
Hello Abderrahim Benmoussa
Thanks so much for your suggestion. I will look at it right away and try getting in touch with them. Can you tell me how much RNA concentration you had in your milk exosomes? Also, how did you estimate its concentration?
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