How can I get rid of much amount of solvent which is needed for the reaction to make film by film applicator?

Indeed, I want to make a very thin film (50 microns) of PU on the steel surface as a coat. for the reaction, much amount of CHCl3 is needed to dissolve one of the reagents to participate in the...

12 October 2020 748 3 View

Does anyone know any reviewer in the field of internal marketing and service innovation ?

I am going to submit my paper in the EI journal and need to introduce four International Experts as a reviewer in the field of marketing. I will appreciate any suggestions. Thank you .

25 February 2020 4,059 7 View

How to remove color of this sample?

Dear Madam/Sirs I have a unknown sample to measure NO2-, PO4 3-, F and NH3. The color was not removed by carbon active and filtration. But when we add AlNO3 the sample color sediment. Do you...

15 January 2020 5,668 3 View

Could you please help us about Atomic absorption PG instrument 500?

We have an AA PG 500 with many problems: 1. The acetylene-air flame is not stable and it turn off after some minutes. 2. The acetylene-nitrous oxide flame is not stable and it turn off after some...

19 October 2019 7,948 2 View

Are there any free online English Language Editing services?

Dear colleagues; Does anyone know a website offering free online English language editing services for scientific manuscripts to be ready for publication? Kind Regards,

21 March 2017 8,832 11 View

Does anyone know what is the transfection efficiency of MCF7 with turbofect reagent?

have seeded 110000 cells in a 24 well plate and transfect cells with 1 migrogram plasmid + 2.5 microlitre of Turbofect. The GFP flourescent as wells as normal picture after 48 hour is attached...

04 September 2015 6,569 6 View

Can H&E staining be used on in-vitro samples?

I am looking for a staining method to detect apoptotic cells vs. necrotics using bright-field microscope (in fact for an easy, quick, and cheap assay!). I know H&E is used for tissue, I would...

21 April 2015 1,655 15 View

Collaborating in writing and publishing a research review paper

I have written a review paper entitled " Role of Internal Marketing in Creating Successful Intercultural Service Encounters: A Literature Review " and got some advice from one of the editor and...

01 January 1970 3,444 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View