Soheila Shokrollahzade

3 Questions 5 Answers 0 Followers

Questions related from Soheila Shokrollahzade

Does anyone know what is the transfection efficiency of MCF7 with turbofect reagent?

have seeded 110000 cells in a 24 well plate and transfect cells with 1 migrogram plasmid + 2.5 microlitre of Turbofect. The GFP flourescent as wells as normal picture after 48 hour is attached...

05 September 2015 6,667 6 View

How can I find the location of proteins to find best method for protein purification?

I want to choose the appropriate purification method.Does anyone know how could I find out that a special protein is membrane protein or cytoplasmic, nuclear.etc?

01 September 2015 8,909 8 View

For subculture of MCF7, Is there any need to count the cells after centrifugation to get the cell density for seeding the cells into the new flask?

I haven't draw the growth curve to know the doubling time.

11 July 2015 5,570 3 View