How can I get rid of much amount of solvent which is needed for the reaction to make film by film applicator?

Indeed, I want to make a very thin film (50 microns) of PU on the steel surface as a coat. for the reaction, much amount of CHCl3 is needed to dissolve one of the reagents to participate in the...

12 October 2020 748 3 View

Does anyone know any reviewer in the field of internal marketing and service innovation ?

I am going to submit my paper in the EI journal and need to introduce four International Experts as a reviewer in the field of marketing. I will appreciate any suggestions. Thank you .

25 February 2020 4,059 7 View

How to remove color of this sample?

Dear Madam/Sirs I have a unknown sample to measure NO2-, PO4 3-, F and NH3. The color was not removed by carbon active and filtration. But when we add AlNO3 the sample color sediment. Do you...

15 January 2020 5,668 3 View

Could you please help us about Atomic absorption PG instrument 500?

We have an AA PG 500 with many problems: 1. The acetylene-air flame is not stable and it turn off after some minutes. 2. The acetylene-nitrous oxide flame is not stable and it turn off after some...

19 October 2019 7,948 2 View

Are there any free online English Language Editing services?

Dear colleagues; Does anyone know a website offering free online English language editing services for scientific manuscripts to be ready for publication? Kind Regards,

21 March 2017 8,832 11 View

Does anyone know what is the transfection efficiency of MCF7 with turbofect reagent?

have seeded 110000 cells in a 24 well plate and transfect cells with 1 migrogram plasmid + 2.5 microlitre of Turbofect. The GFP flourescent as wells as normal picture after 48 hour is attached...

04 September 2015 6,569 6 View

For subculture of MCF7, Is there any need to count the cells after centrifugation to get the cell density for seeding the cells into the new flask?

I haven't draw the growth curve to know the doubling time.

10 July 2015 5,455 3 View

Can H&E staining be used on in-vitro samples?

I am looking for a staining method to detect apoptotic cells vs. necrotics using bright-field microscope (in fact for an easy, quick, and cheap assay!). I know H&E is used for tissue, I would...

21 April 2015 1,655 15 View

Collaborating in writing and publishing a research review paper

I have written a review paper entitled " Role of Internal Marketing in Creating Successful Intercultural Service Encounters: A Literature Review " and got some advice from one of the editor and...

01 January 1970 3,444 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View