How to find load / force acting on the top face in ansys workbench?

The image attached is the representation of my application. This is an assembly of three items (top plate, bottom plate and the 5 load cells). The bottom plate is fixed and a load of 40 Kg is...

15 December 2020 1,145 6 View

What is the best deep learning technique for detection of Adverse Drug Reaction from EHR?

We are interested to find the best deep learning technique for detection of Adverse Drug Reaction.

03 October 2020 3,513 2 View

Can we choose multiple natural sources together for the synthesis of Carbon quantum dots?

will it be an advantage for the synthesis ?

26 August 2019 2,223 3 View

Flow separation : Turbulence or Laminar?

Hi researchers, I am performing CFD analysis inside an electronics enclosure. The Reynolds number is below 1000 but I have some blocks and heat sinks inside that creates flow separation and wake...

20 May 2019 1,357 19 View

Has anyone experienced varied behaviour of ICP-OES instrument each time it is operated?

We face the following problem: 1) It's a general problem faced. During the standard calibration (irrespective of metal), the std. calibration was successful the previous time, but the next time...

24 January 2018 2,469 3 View

What is the flaky material present on the surface of the biomass (Untreated) as seen in the Scanning Electron Micrograph?

23 June 2017 3,864 1 View

Is it advisable to determine the Cr(VI) concentration present in NaOH (Desorbing agent) using 1,5 Diphenyl carbazide method?

06 February 2017 8,277 3 View

I would like to know if anyone has experienced infecting T47D (Breast Cancer) cells lines using Lentivirus?

Lentivirus; T47D cells

10 May 2016 1,308 3 View

What is the best method to screen an efficient part- root, shoot and leaves (separately)of a plant biomass in metal uptake?

With the optimal pH found, can I screen the part (root, shoot or leaf) by just subjecting it to the metal solution (fixed conc.) at optimum pH for a particular time period? Or is time studies...

12 March 2016 9,696 3 View

Can anyone suggest a data base to identify the protein of the MDA-MB 231 cell line (breast cancer)?

Name of protein to be chosen for MDA_MB231 cell lines and its structure will be useful.

16 February 2016 7,370 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View