In this article you have more details about how to differentiate this two bacterials

Dear Akhmad,

I would check the smelling and the growing temperatur:

The smelling of P. aeruginosa is sweet like drops. P. fluorescens smelling is putrid.

P. aeruginosa grows at a 42°C. P.fluorescens prefers low temperatur like 30°C. It don´t grows at 42°C.

Dear Akhmad,

Look at Bergey’s Manual of Systematic Bacteriology.

To differentiate them: P. aeruginosa produces pyocyanin (bleu pigment), can grow at 42°C and is resistant to Kanamycin.

Good luck

Better is to sequence their genome, it might not be the species you think they are.

thanks for all. nice to meet you :)

Oana alexandra@: ok. I have eksploration pseudomonas and right now I have found some bacteria, one of their colony look glow green on kings B agar. I Think it is pseudomonas fluorescens or pseudomonas aeruginosa, so i need your suggestion or method to identification and distinguish both.

Heidelinde and Itto Maroi, thanks for your answer, I like it :)

if you ready, I need the more detail of protocol to identification pseudomonas

Thanks very much to Bhoj R singh for your attachment.  I will learn your article... :)

No Característica P. fluorescens P. aeruginosa

1 Forma celular Bacilo aeróbico Bacteria aeróbica

2 Metabolismo Saprofito Patógeno oportunista

3 Reacción de Gram Gram negativo Gram negativo

4 Flagelos Flagelos polares Flagelos monopolares

5 Pigmento Fluoresceína Piocianina, fluoresceína, piorrubina

6 Medio de cultivo Medio mineral NH4+ o NO3-, Hidrocarburos

7 Relación con las plantas Estimula el crecimiento vegetal Produce putrefacción de raíces

8 Metabolitos Produce antibióticos Toxina A

9 Identificación Fluoresceína Apariencia perlada y color a uvas. Pigmentos piocianina y fluoresceína, habilidad de crecer a 42°C

Miguel gualoto@: thanks for your answer. but i am not understand your language. may be english is better. 

The methods that others are sugesting are based on traits that can be shared by hundreds of other bacteria, this may let you to wrongly identify the bacteria.

Best is to isolate its 16S rDNA and sequence it. This way you will be able to identify your bacteria with 100% of accuracy.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209952/

Thanks Mr. Jose Luis Brioso :)

Cultural distinction between Pseudomonas fluorescens and P. aeruginosa

is incubation temperature, P. aeruginosa  can grow at 42C

P. flourescens  cannot and does better at 30 C (

Completely agree with Dr. José Luis however not always available techniques are so expensive at least in my country and therefore a simple way to approach to differentiate in the laboratory is to place a brain heart infusion broth with the inoculate suspect and place in water bath at 42 ° C if growth in 24 hours microorganism is P. aeruginosa, also in the susceptibility can be attached 1 disc kanamycin and carbenicillin according to one of Koneman et al P. aeruginosa is resistant to kanamycin and sensitive carbenicillin while P. fluorescens is the opposite.

I hope to help

Sorry, my english is very bad

The easiest way to distinguish between P. aeruginosa and P. fluorescens is by culturing at 37 and 30C. P. fluorescens does not grow at 37, P. aeruginosa will grow at both t

Olga Zaborina and all, thanks for your answer :)

 mariesa gill@: thanks for your answer...

 this is the culture of pseudomonas on King 'B Agar Medium, how about your opinion?

can be easily distinguished on the basis of the temperature at which they grow

Grow plates at 37C and 30C. Only P. aeruginosa will grow at 37C

With all due respects to José Luis Brioso Jiménez, 16s RNA gene sequencing will not help here. P. aeruginosa and P. fluorescens are so closely related that they are not distinguishable by 16s sequence. Use one of the phenotypic test suggested.