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Questions related from Andrew Jenkins
It is a well-known fact that if a qPCR sample is partially inhibitory, the log-transformed amplification curve will be flattened. This can be deduced from first principals, but I am unable to find...
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I am interested in producing videos of growing and dividing bacterial cells. The problem, as I see it, it to keep the cells still and well-oxygenated without them drying out. I have tried using a...
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I'm having difficulties with VRB agar. The instruction on the bottle say heat it in a boiling water bath - but this takes all day, at least with a 500 ml bottle. I don't like to microwave agar as...
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We are doing experiments on growth of bacteria with plastic as the sole carbon source. We use M9 minimal salts, but I am concerned that it may not be sufficient as it contains only major...
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We are doing experiments with wild-type bacteriophage lambda. Plaque counting is an essential part of the work. Unfortunately, the plaques are rather small and indistinct, which makes it all but...
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I am trying to assemble a collection of E. coli plasmids of various incompatibility groups for a project on conjugative pili. Can anyone suggest a source to contact. I've tried CGSC, but I didn't...
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We are doing some experiments on plastic-degrading microbes, growing them on M9 salts with plastic as the sole carbon source. We buy the M9 salts from Sigma. According to the formulation, the...
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We are trying to get DNA out of microalgae, but we can't get them to lyse. We suspect this is because the slime capsule is preventing cellulase from reaching the cell wall. Does anyone have any...
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Read 1 answer by scientists to the question asked by Andrew Jenkins on Dec 10, 2014
10 December 2014 9,539 1 View
We are working with a couple of plasmids which are rather difficult to isolate in satisfactory quantities. We tried chloramphenicol amplification on one of them and this made a huge difference and...
21 February 2014 1,131 4 View
We are having trouble transforming plasmids into clinical strains of E. coli by electroporation. We use a 1500 V pulse and standard electroporation buffer and follow the protocol that comes with...
21 February 2014 9,601 3 View
