Label synapse by spontaneous FM dye incubation?

Dear all, I am working on real time imaging of cultured neurons. Currently, I want to distinguish presynaptic boutons from extra-synaptic areas in live-cell imaging. My advisor suggest to incubate...

03 April 2017 1,520 4 View

Live-imaging for synaptogenesis?

Hi guys, I am working on live-cell imaging of primary cultured neurons. I want to identify the formation of synapses in my live-imaging. I doubt the transfection of synaptic markers as they may go...

06 July 2016 8,491 7 View

How can I screen an unknown partner of a known protein?

Hi guys, I am looking for a binding partner for my molecule. It should be a new one as no one knows what it is. I feel that there should have a binding partner. But it is just a kind of...

11 December 2015 4,705 7 View

Does antibiotics affect the efficiency for Ca transfection?

Dear all, I am straggling with Ca transfection on neurons. I heared that antibiotics like Penicillin will reduce the transfection efficiency. My transfection medium do not contain antibiotics, but...

03 April 2015 5,690 4 View

Is there any antagonist or agonist for TrkB.T1?

Dear all, I am working on a truncated TrkB receptor, TrkB.T1. Now, I need to increase or inhibit its activity in cultured neurons. Do you guys know any good agonist or activator for TrkB.T1? Or,...

02 March 2015 3,612 2 View

How can I test for protein expression only in neurons?

I would like to quantify the expression of a protein in neurons. However, it is also highly expressed in glia. How can I exclude the glia protein expression? I mean to test the protein levels...

02 March 2015 6,950 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View